Klebsiella pneumoniae is a nosocomial agent during nosocomial outbreaks. Many plant oils have antimicrobial activities and have been studied as natural sources to fight many antibiotics.
This study aims to investigate the antibacterial and antibiotic effects of the nano-sized preparations of rosemary and ginger essential oils on the clinical isolates of K. pneumonia resistant to colistin.
The isolation and determination of 30 strains of K. pneumonia isolated from different human samples was done, then tested for antibiotic sensitivity and detecting biofilm genes.
The testing of the activity of the tested essential oils and their chitosan nanoparticle formulas against selected isolates was performed by determining their MICs by the broth dilution method, after it was a real-time quantitative PCR and biofilm inhibition test for expression of mrkD gene in the presence of oil and nanoparticle formulation compared with untreated bacterial isolates.
Our results show that the minimum inhibitory concentration of rosemary oil and ginger oil is 1250 μg / ml, the rosemary oil with the nanostructured and the ginger oil with the nanostructured is 625 μg / ml and the rosemary oil contains chitosan nanoparticles and ginger oil. chitosan nanoparticles is 156 μg / ml.
The results also showed complete inhibition of mrkD gene expression when compared with K. pneumonia without treatment. We can conclude that the oil-loaded chitosan nanoparticles showed high antibacterial and antibiotic activity.
